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Selective deletion of Keap1 in Clara cells in mouse lungs elevated the expression of Nrf2-dependent genes ( Nqo1 and Gclm ), increased the total level of glutathione in the lungs, protected Clara cells against oxidative stress ex vivo, and protected the lungs against oxidative stress and CS-induced inflammation in intact animals (138)

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Transcriptomic analysis RNA extraction, quality control, library preparation, and sequencing Total RNA was extracted using TRIzol reagent (Invitrogen, Thermo Fisher Scientific, USA) or the RNeasy Mini Kit (Qiagen, Germany), following the manufacturers instructions

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